Activation of bombesin receptor subtype-3 stimulates adhesion of lung cancer cells

Lung Cancer. 2006 Nov;54(2):143-8. doi: 10.1016/j.lungcan.2006.08.005. Epub 2006 Sep 18.


Bombesin receptor subtype-3 (BRS-3) is an orphan G protein-coupled receptor having sequence homologies to gastrin-releasing peptide and neuromedin B receptors. [d-Phe6, beta-Ala11, Phe13, Nle14]bombesin(6-14) is known to act as a synthetic receptor agonist for BRS-3. To characterize BRS-3-mediated biological responses, we examined the effect of BRS-3 activation by [d-Phe6, beta-Ala11, Phe13, Nle14]Bn(6-14) on the adhesion of the small cell lung cancer NCI-N417 cells that express native BRS-3. We found that the BRS-3 agonist stimulated adhesion of NCI-N417 cells in laminin-coated culture wells. The adhesion of the cells to laminin induced by BRS-3 activation was accompanied by an increase in vinculin-like immunoreactivity and diminished in the presence of an anti-beta1 integrin antibody, suggesting that the receptor activation stimulates focal adhesion formation. We suggest that BRS-3 may be involved in invasion and metastasis of certain cancer cells, like small cell lung cancer cells, upon attachment to laminin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bombesin / analogs & derivatives
  • Bombesin / pharmacology
  • Carcinoma, Small Cell / chemistry
  • Carcinoma, Small Cell / metabolism*
  • Cell Adhesion*
  • Cell Line, Tumor
  • Focal Adhesions / drug effects
  • Focal Adhesions / metabolism*
  • Humans
  • Integrin beta1 / analysis
  • Laminin / metabolism
  • Lung Neoplasms / chemistry
  • Lung Neoplasms / metabolism*
  • Oligopeptides / pharmacology
  • Peptide Fragments / pharmacology
  • Peptides, Cyclic
  • Receptors, Bombesin / metabolism*
  • Somatostatin / analogs & derivatives
  • Somatostatin / pharmacology
  • Vinculin / analysis


  • (phenylalanyl(6)-alanyl(11)-phenylalanyl(13)-Nle(14))Bn(6-14)
  • Integrin beta1
  • Laminin
  • Oligopeptides
  • Peptide Fragments
  • Peptides, Cyclic
  • Receptors, Bombesin
  • bombesin receptor subtype 3
  • lanreotide
  • Vinculin
  • Somatostatin
  • Bombesin