Degradation of fluorobenzene by Rhizobiales strain F11 via ortho cleavage of 4-fluorocatechol and catechol

Appl Environ Microbiol. 2006 Nov;72(11):7413-7. doi: 10.1128/AEM.01162-06. Epub 2006 Sep 15.

Abstract

The aerobic metabolism of fluorobenzene by Rhizobiales sp. strain F11 was investigated. Liquid chromatography-mass spectrometry analysis showed that 4-fluorocatechol and catechol were formed as intermediates during fluorobenzene degradation by cell suspensions. Both these compounds, unlike 3-fluorocatechol, supported growth and oxygen uptake. Cells grown on fluorobenzene contained enzymes for the ortho pathway but not for meta ring cleavage of catechols. The results suggest that fluorobenzene is predominantly degraded via 4-fluorocatechol with subsequent ortho cleavage and also partially via catechol.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alphaproteobacteria / enzymology
  • Alphaproteobacteria / growth & development
  • Alphaproteobacteria / metabolism*
  • Biodegradation, Environmental
  • Catechols / chemistry
  • Catechols / metabolism*
  • Culture Media
  • Fluorobenzenes / metabolism*
  • Oxygen Consumption
  • Substrate Specificity

Substances

  • Catechols
  • Culture Media
  • Fluorobenzenes
  • 4-fluorocatechol
  • catechol