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, 12 (33), 5344-51

Alteration of Peripheral Blood Lymphocyte Subsets in Acute Pancreatitis

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Alteration of Peripheral Blood Lymphocyte Subsets in Acute Pancreatitis

Miroslawa Pietruczuk et al. World J Gastroenterol.

Abstract

Aim: To evaluate peripheral blood lymphocyte subsets in patients with acute pancreatitis (AP).

Methods: Twenty patients with mild AP (M-AP) and 15 with severe AP (S-AP) were included in our study. Peripheral blood lymphocytes were examined at d 1-3, 5, 10 and 30 by means of flow cytometry.

Results: A significant depletion of circulating lymphocytes was found in AP. In the early AP, the magnitude of depletion was similar for T- and B- lymphocytes. In the late course of S-AP, B-lymphocytes were much more depleted than T-lymphocytes. At d 10, strong shift in the CD7+/CD19+ ratio implicating predominance of T- over B-lymphocytes in S-AP was found. Among T-lymphocytes, the significant depletion of the CD4+ population was observed in M-AP and S-AP, while CD8+ cells were in the normal range. Lymphocytes were found to strongly express activation markers: CD69, CD25, CD28, CD38 and CD122. Serum interleukin-2 (IL-2), IL-4, IL-5, IL-10, interferon-gamma (IFN-gamma) and tumor necrosis factor-alpha (TNF-alpha) levels were significantly increased in both forms of AP. The magnitude of elevation of cytokines known to be produced by Th2 was much higher than cytokines produced by Th1 cells.

Conclusion: AP in humans is characterized by significant reduction of peripheral blood T- and B-lymphocytes.

Figures

Figure 1
Figure 1
Peripheral blood lymphocytes in patients with AP. A: Total count; B: CD7+; C: CD19+; D: CD4+; E: CD8+; F: CD69+; G: CD25+. Results are expressed as mean ± SD. aP < 0.05 vs control; cP < 0.05 vs mild AP at the same day of observation.
Figure 2
Figure 2
Peripheral blood T-lymphocytes (CD7+) (A) and B-lymphocytes (CD19+) (B) expressing CD122 in patients with AP. Results are expressed as mean ± SD. aP < 0.05 vs control; cP < 0.05 vs mild AP at the same day of observation.
Figure 3
Figure 3
Peripheral blood CD8+ cells expressing CD38 (A) and CD28 (B) in patients with AP. Results are expressed as mean ± SD. aP < 0.05 vs control; cP < 0.05 vs mild AP at the same day of observation.
Figure 4
Figure 4
Markers of lymphocyte apoptosis in patients with AP. Peripheral blood lymphocytes expressing CD95 (A), CD3+ lymphocytes expressing Annexin V (B) and CD19+ lymphocytes expressing Annexin V (C). Results are expressed as mean ± SD. aP < 0.05 vs control; cP < 0.05 vs mild AP at the same day of observation.

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