Inhibition of serotonin uptake into mouse brain synaptosomes by ionophores and ion-channel agents

Brain Res. 1990 Jun 25;521(1-2):347-51. doi: 10.1016/0006-8993(90)91565-x.


[3H]Serotonin uptake into mouse cerebrocortical synaptosomes was decreased by the K+ ionophore valinomycin, the K+ and Na+ ionophore gramicidin, and the proton ionophore carbonylcyanide m-chlorophenylhydrazone. The Na+/H+ exchanger monensin reduced uptake at non-depolarizing concentrations. Uptake was also decreased by inhibition of the Na+, K(+)-ATPase with ouabain and by tetrodotoxin-sensitive activation of voltage-dependent sodium channels with veratridine, batrachotoxin and scorpion venom. In contrast, the Ca2+ channel agents BAY K8644 and nimodipine were ineffective. The effect of reducing the Na+ gradient depended upon whether the internal Na+ concentration was raised (i.e. by scorpion venom, monensin) or the external Na+ concentration was lowered (37 mM NaCl in the medium).

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cerebral Cortex / drug effects
  • Cerebral Cortex / metabolism*
  • Gramicidin / pharmacology*
  • Ion Channels / drug effects
  • Ion Channels / physiology*
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Monensin / pharmacology*
  • Potassium Channels / drug effects
  • Potassium Channels / physiology
  • Serotonin / pharmacokinetics*
  • Sodium Channels / drug effects
  • Sodium Channels / physiology
  • Synaptosomes / drug effects
  • Synaptosomes / metabolism*
  • Valinomycin / pharmacology*


  • Ion Channels
  • Potassium Channels
  • Sodium Channels
  • Gramicidin
  • Valinomycin
  • Serotonin
  • Monensin