Effect of 5-aza-2'-deoxycytidine and retinoic acid on differentiation and c-myc expression in HL-60 myeloid leukemic cells

Cancer Lett. 1990 Oct 8;54(1-2):21-8. doi: 10.1016/0304-3835(90)90086-d.


The antineoplastic effects of 5-aza-2'-deoxycytidine (5-AZA-CdR) and retinoic acid on human HL-60 myeloid leukemic cells were investigated. 5-AZA-CdR or retinoic acid reduced the clonogenicity and induced the differentiation of HL-60 leukemic cells. The effects produced by 5-AZA-CdR and retinoic acid in combination on clonogenicity and differentiation of HL-60 leukemic cells were additive. These agents in combination also produced an additive decrease in the mRNA expression of the c-myc. These data indicate that 5-AZA-CdR and retinoic acid in combination produce an additive antineoplastic effect against HL-60 human myeloid leukemic cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents*
  • Azacitidine / analogs & derivatives*
  • Azacitidine / pharmacology
  • Cell Differentiation / drug effects
  • Cell Division / drug effects
  • Decitabine
  • Drug Synergism
  • Gene Expression / drug effects*
  • Humans
  • Proto-Oncogene Proteins c-myc / metabolism*
  • Tretinoin / pharmacology*
  • Tumor Cells, Cultured / drug effects


  • Antineoplastic Agents
  • Proto-Oncogene Proteins c-myc
  • Tretinoin
  • Decitabine
  • Azacitidine