Identification of a ligand for the c-kit proto-oncogene

Cell. 1990 Oct 5;63(1):167-74. doi: 10.1016/0092-8674(90)90297-r.


We report the purification and N-terminal amino acid sequence of a novel mast cell growth factor, termed MGF, from the supernatants of a murine stromal cell line. A panel of interleukin 3-dependent cell lines were screened for responsiveness to partially purified MGF in [3H]thymidine incorporation assays; proliferative stimulation of these cells in response to MGF correlated with expression of mRNA for the c-kit protooncogene. MGF was shown to be a ligand for c-kit by cross-linking 125I-labeled MGF to c-kit-expressing cells with subsequent immunoprecipitation of the complex with antiserum specific for the C-terminus of c-kit. This establishes MGF as a ligand for the c-kit protein.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cell Division
  • Cell Line
  • DNA Replication
  • Hematopoietic Cell Growth Factors / genetics*
  • Hematopoietic Cell Growth Factors / isolation & purification
  • Hematopoietic Cell Growth Factors / metabolism
  • Ligands
  • Molecular Sequence Data
  • Protein-Tyrosine Kinases / genetics
  • Proto-Oncogene Proteins / genetics*
  • Proto-Oncogene Proteins c-kit
  • Proto-Oncogenes*
  • RNA, Messenger / genetics
  • Stem Cell Factor
  • Transcription, Genetic


  • Hematopoietic Cell Growth Factors
  • Ligands
  • Proto-Oncogene Proteins
  • RNA, Messenger
  • Stem Cell Factor
  • Protein-Tyrosine Kinases
  • Proto-Oncogene Proteins c-kit