Construction of plasmids for RNA interference and in vitro transcription of double-stranded RNA

Methods Mol Biol. 2006;351:109-17. doi: 10.1385/1-59745-151-7:109.

Abstract

Double-stranded RNA (dsRNA)-induced gene silencing in Caenorhabditis elegans involves the manufacture and delivery of defined sequences of dsRNA to the organism, followed by a careful monitoring for loss-of-function phenocopies in treated animals. In this chapter, we describe how to generate DNAs that can be used as templates for transcription of dsRNA.

Publication types

  • Review

MeSH terms

  • Animals
  • Caenorhabditis elegans / genetics*
  • Cell-Free System
  • Genes, Helminth / genetics*
  • RNA Interference*
  • RNA, Double-Stranded / genetics*
  • RNA, Double-Stranded / pharmacology
  • Transcription, Genetic*

Substances

  • RNA, Double-Stranded