Imaging diacylglycerol dynamics at organelle membranes

Nat Methods. 2006 Oct;3(10):797-9. doi: 10.1038/nmeth930.


Fluorescence imaging is a powerful technique to visualize spatiotemporal dynamics of biomolecules in living cells. We describe fluorescent indicators for a lipid second messenger, diacylglycerol (DAG), which allow the localized analysis of DAG dynamics at subcellular membranes. We have thus pinpointed that DAG concentrations increase and/or decrease at not only the plasma membrane but also organelle membranes such as endomembranes and mitochondrial outer membranes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Diglycerides / chemistry*
  • Dogs
  • Fluorescent Dyes / chemistry*
  • Intracellular Membranes / physiology*
  • Microscopy, Fluorescence / methods
  • Mitochondrial Membranes / physiology*


  • Diglycerides
  • Fluorescent Dyes