Augmentation of natural killer cell and antibody-dependent cellular cytotoxicity in BALB/c mice by sulforaphane, a naturally occurring isothiocyanate from broccoli through enhanced production of cytokines IL-2 and IFN-gamma

Immunopharmacol Immunotoxicol. 2006;28(3):443-57. doi: 10.1080/08923970600928049.

Abstract

Effect of sulforaphane on cell-mediated immune (CMI) response was studied in normal as well as Ehrlich ascites tumor-bearing BALB/c mice. Administration of sulforaphane significantly enhanced natural killer (NK) cell activity in both normal as well as tumor-bearing animals, and the activity was observed earlier than in tumor-bearing control animals. Antibody-dependent cellular cytotoxicity (ADCC) also was enhanced significantly in both normal as well as tumor-bearing animals after sulforaphane administration compared with untreated control tumor-bearing animals. An early antibody-dependent complement-mediated cytotoxicity (ACC) also was observed in sulforaphane-treated normal and tumor-bearing animals. Administration of sulforaphane significantly enhanced the production of Interleukin-2 and Interferon-gamma in normal as well as tumor-bearing animals. In addition, sulforaphane significantly enhanced the proliferation of splenocytes, bone marrow cells, and thymocytes by stimulating the mitogenic potential of various mitogens such as concanavalin A, phytohaemagglutinin, poke weed mitogen, and lipopolysaccharide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibody-Dependent Cell Cytotoxicity / drug effects*
  • Antibody-Dependent Cell Cytotoxicity / immunology
  • Bone Marrow Cells / drug effects
  • Brassica / chemistry*
  • Carcinoma, Ehrlich Tumor / drug therapy*
  • Carcinoma, Ehrlich Tumor / immunology
  • Carcinoma, Ehrlich Tumor / metabolism
  • Cell Proliferation / drug effects
  • Complement Activation / drug effects
  • Complement Activation / immunology
  • Cytokines / biosynthesis
  • Cytokines / blood
  • Drug Synergism
  • Humans
  • Injections, Intraperitoneal
  • Interferon-gamma / metabolism
  • Interleukin-2 / metabolism
  • Isothiocyanates / administration & dosage
  • Isothiocyanates / chemistry
  • Isothiocyanates / pharmacology
  • K562 Cells
  • Killer Cells, Natural / drug effects*
  • Killer Cells, Natural / immunology
  • Killer Cells, Natural / metabolism
  • Lymphocyte Activation / drug effects
  • Lymphocyte Activation / immunology
  • Mice
  • Mice, Inbred BALB C
  • Mitogens / pharmacology
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology
  • Spleen / cytology
  • Spleen / drug effects
  • T-Lymphocytes / drug effects
  • T-Lymphocytes / immunology
  • T-Lymphocytes / metabolism
  • Thiocyanates / administration & dosage
  • Thiocyanates / chemistry
  • Thiocyanates / pharmacology*
  • Thymus Gland / cytology
  • Thymus Gland / drug effects

Substances

  • Cytokines
  • Interleukin-2
  • Isothiocyanates
  • Mitogens
  • Plant Extracts
  • Thiocyanates
  • isothiocyanic acid
  • Interferon-gamma
  • sulforafan