EGCG protects HT-22 cells against glutamate-induced oxidative stress

Neurotox Res. 2006 Aug;10(1):23-30. doi: 10.1007/BF03033331.


(-)-Epigallocatechin-3-gallate (EGCG) is a major polyphenol in green tea. Many health promoting effects of EGCG have been reported based on its antioxidative and gene modulation properties, but no study has demonstrated a protective effect of EGCG against glutamate-induced neuronal damage. Excessive glutamate stimulation on neuronal cells leads to accumulation of reactive oxygen species (ROS) which ultimately contribute to cell death in stroke, trauma and other neurodegenerative disorders. In this study, mouse hippocampal cell line, HT-22, was used to determine the effect of EGCG on glutamate neurotoxicity. It was found that EGCG protected HT-22 cells against glutamate neurotoxicity when administered 10 h after glutamate incubation. The protective action of EGCG is mainly due to its antioxidative effect.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / pharmacology*
  • Catechin / analogs & derivatives*
  • Catechin / pharmacology
  • Cell Line, Transformed
  • Cell Survival / drug effects
  • Coculture Techniques / methods
  • Dose-Response Relationship, Drug
  • Drug Interactions
  • Gene Expression Regulation / drug effects
  • Glutamic Acid / pharmacology*
  • Heme Oxygenase-1 / genetics
  • Heme Oxygenase-1 / metabolism
  • Hippocampus / cytology
  • Mice
  • Oxidative Stress / drug effects*
  • RNA, Messenger / metabolism
  • Reactive Oxygen Species / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction / methods
  • Time Factors


  • Antioxidants
  • RNA, Messenger
  • Reactive Oxygen Species
  • Glutamic Acid
  • Catechin
  • epigallocatechin gallate
  • Heme Oxygenase-1