Mitogen-activated protein kinase signaling is essential for activity-dependent dendritic protein synthesis

Neuroreport. 2006 Oct 23;17(15):1575-8. doi: 10.1097/01.wnr.0000234742.42818.ff.


The expression of long-lasting synaptic plasticity requires synthesis of new proteins. A critical locus for protein synthesis to support synaptic plasticity is the dendrites. Previous studies demonstrate that synaptic activity activates dendritic protein synthesis. The mechanism by which synaptic activity stimulates protein synthesis in dendrites is, however, poorly understood. This study is to determine the role of the mitogen-activated protein kinase signaling pathway in activity-dependent dendritic protein synthesis. Using a green fluorescent protein reporter with CaMKII 5' and 3'untranslated regions, we show that dendritic synthesis of the green fluorescent protein induced by N-methyl-D-aspartate stimulation is abolished by U0126, a specific inhibitor of mitogen-activated protein kinase signaling. Our results suggest an important role of the mitogen-activated protein kinase signaling in dendritic protein synthesis induced by N-methyl-D-aspartate receptor activation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anisomycin / pharmacology
  • Butadienes / pharmacology
  • Cells, Cultured
  • Dendrites / drug effects
  • Dendrites / metabolism*
  • Drug Interactions
  • Embryo, Mammalian
  • Enzyme Activation / drug effects
  • Enzyme Inhibitors / pharmacology
  • Excitatory Amino Acid Agonists / pharmacology
  • Green Fluorescent Proteins / metabolism
  • Hippocampus / cytology
  • Mice
  • Mitogen-Activated Protein Kinases / metabolism*
  • N-Methylaspartate / pharmacology
  • Neurons / cytology
  • Nitriles / pharmacology
  • Protein Biosynthesis / drug effects
  • Protein Biosynthesis / physiology*
  • Protein Synthesis Inhibitors / pharmacology
  • Signal Transduction / drug effects
  • Signal Transduction / physiology*
  • Time Factors
  • Transfection / methods


  • Butadienes
  • Enzyme Inhibitors
  • Excitatory Amino Acid Agonists
  • Nitriles
  • Protein Synthesis Inhibitors
  • U 0126
  • Green Fluorescent Proteins
  • N-Methylaspartate
  • Anisomycin
  • Mitogen-Activated Protein Kinases