Insulin regulates the expression of the insulin-like growth factor binding protein 2 mRNA in rat hepatocytes

Mol Endocrinol. 1990 Sep;4(9):1320-6. doi: 10.1210/mend-4-9-1320.


The goal of this study was to find out whether GH or insulin regulate the mRNA expression of the fetal binding protein of insulin-like growth factor (IGFBP-2). Primary hepatocytes from adult rats were used as a test system. IGFBP-2 mRNA was abundant in cells cultured in the absence of hormones and markedly reduced in cultures containing insulin. Addition of GH had no effect on IGFBP-2 mRNA levels although the cells are responsive to GH as demonstrated by a GH mediated elevation of IGF l mRNA levels. Half-maximal down-regulation of IGFBP-2 mRNA levels occurred at an insulin concentration of 1 to 2 x 10(-10) M. The finding that insulin is a potent negative regulator of hepatic IGFBP-2 mRNA levels suggests a physiologically important regulatory link between the two hormones insulin and IGF l.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carrier Proteins / genetics*
  • Cells, Cultured
  • Dose-Response Relationship, Drug
  • Gene Expression Regulation / drug effects*
  • Growth Hormone / pharmacology
  • Insulin / administration & dosage
  • Insulin / pharmacology*
  • Insulin-Like Growth Factor Binding Proteins
  • Liver / metabolism*
  • Male
  • Nucleic Acid Hybridization
  • RNA, Messenger / metabolism*
  • Rats
  • Rats, Inbred Strains


  • Carrier Proteins
  • Insulin
  • Insulin-Like Growth Factor Binding Proteins
  • RNA, Messenger
  • Growth Hormone