Isolation of an endogenously processed immunodominant viral peptide from the class I H-2Kb molecule

Nature. 1990 Nov 15;348(6298):213-6. doi: 10.1038/348213a0.


Using an approach for isolating and characterizing peptide fractions that are intracellularly associated with major histocompatibility complex class I molecules, the major peptide recognized by cytotoxic T cells specific for the vesicular stomatitis virus has been isolated from the H-2Kb molecule of infected cells. This endogenously processed octapeptide is allele-specific as it does not bind to H-2Db molecules, and contains the core sequence of the epitope of the nucleocapsid protein of the vesicular stomatitis virus identified by testing with exogenous synthetic peptides.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, Viral / chemistry
  • Antigens, Viral / immunology
  • Antigens, Viral / isolation & purification
  • Capsid* / chemistry
  • Capsid* / immunology
  • Cell Line
  • Chromatography, High Pressure Liquid
  • Epitopes / chemistry
  • Epitopes / immunology
  • Epitopes / isolation & purification
  • H-2 Antigens / analysis*
  • Histocompatibility Antigens Class I / analysis*
  • Mice
  • Molecular Sequence Data
  • Molecular Weight
  • Vesicular stomatitis Indiana virus* / immunology
  • Viral Core Proteins / chemistry
  • Viral Core Proteins / immunology
  • Viral Core Proteins / isolation & purification*


  • Antigens, Viral
  • Epitopes
  • H-2 Antigens
  • H-2Kb protein, mouse
  • Histocompatibility Antigens Class I
  • Viral Core Proteins