Histamine acts directly on calcitonin gene-related peptide- and substance P-containing trigeminal ganglion neurons as assessed by calcium influx and immunocytochemistry

Neurosci Lett. 1990 Jul 31;115(2-3):171-6. doi: 10.1016/0304-3940(90)90450-n.


Primary cultures of rat trigeminal ganglion cells were exposed to histamine, and the intracellular free-calcium concentration, [Ca2+]i, was measured by the calcium-sensitive dye fura-2. Histamine increased the [Ca2+]i of the neurons. Pretreatment of the cells with histamine H1-receptor blocker, or removal of extracellular calcium, abolished the response, however, the response was not altered by pretreatment with H2-blocker. Immunocytochemical analysis showed that these cultured cells that responded to histamine identically showed substance P- or calcitonin gene-related peptide-like immunoreactivity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcitonin Gene-Related Peptide / metabolism*
  • Calcium / pharmacokinetics*
  • Cells, Cultured
  • Cimetidine / pharmacology
  • Fura-2
  • Histamine / pharmacology*
  • Immunohistochemistry
  • Pyrilamine / pharmacology
  • Rats
  • Rats, Inbred Strains
  • Receptors, Histamine / drug effects
  • Receptors, Histamine / physiology*
  • Substance P / metabolism*
  • Trigeminal Ganglion / cytology
  • Trigeminal Ganglion / drug effects
  • Trigeminal Ganglion / metabolism*


  • Receptors, Histamine
  • Substance P
  • Cimetidine
  • Histamine
  • Pyrilamine
  • Calcitonin Gene-Related Peptide
  • Calcium
  • Fura-2