Molecular cloning and functional expression of the cardiac sarcolemmal Na(+)-Ca2+ exchanger

Science. 1990 Oct 26;250(4980):562-5. doi: 10.1126/science.1700476.


The Na(+)-Ca2+ exchanger of the cardiac sarcolemma can rapidly transport Ca2+ during excitation-contraction coupling. To begin molecular studies of this transporter, polyclonal antibodies were used to identify a complementary DNA (cDNA) clone encoding the Na(+)-Ca2+ exchanger protein. The cDNA hybridizes with a 7-kilobase RNA on a Northern blot and has an open reading frame of 970 amino acids. Hydropathy analysis suggests that the protein has multiple transmembrane helices, and a small region of the sequence is similar to that of the Na(+)- and K(+)-dependent adenosine triphosphatase. Polyclonal antibodies to a synthetic peptide from the deduced amino acid sequence react with sarcolemmal proteins of 70, 120, and 160 kilodaltons on immunoblots. RNA, synthesized from the cDNA clone, induces expression of Na(+)-Ca2+ exchange activity when injected into Xenopus oocytes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Triphosphate / pharmacology
  • Amino Acid Sequence
  • Animals
  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics*
  • Chemical Phenomena
  • Chemistry, Physical
  • Cloning, Molecular*
  • DNA / genetics
  • DNA Probes
  • Dogs
  • Gene Expression*
  • Glycosylation
  • Immunoblotting
  • Molecular Sequence Data
  • Molecular Weight
  • Myocardium / chemistry*
  • Nucleic Acid Hybridization
  • RNA / genetics
  • RNA, Complementary
  • Sarcolemma / chemistry*
  • Sodium-Calcium Exchanger


  • Carrier Proteins
  • DNA Probes
  • RNA, Complementary
  • Sodium-Calcium Exchanger
  • RNA
  • Adenosine Triphosphate
  • DNA

Associated data

  • GENBANK/M57523