Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2007 Jan;203(1):123-7.
doi: 10.1016/j.expneurol.2006.07.028. Epub 2006 Sep 27.

Recombinant Adenovirus Vector-Mediated Functional Expression of neurotropin-3 Receptor (TrkC) in Neural Stem Cells

Affiliations

Recombinant Adenovirus Vector-Mediated Functional Expression of neurotropin-3 Receptor (TrkC) in Neural Stem Cells

Jun-Mei Wang et al. Exp Neurol. .

Abstract

We have constructed a recombinant adenovirus expression vector carrying the human neurotrophin-3 (NT-3) receptor TrkC (tyrosine protein kinase C) gene (rAd-TrkC; 2478 bp) and confirmed the expression of the encoded TrkC in green fluorescent protein (GFP)-murine neural stem cells (NSCs) by reverse transcription polymerase chain reaction (RT-PCR), Western blot analysis, and immunocytochemistry. The activity of the expressed rAd-TrkC was verified in vitro by evaluating dose-related responses of NSCs to NT-3, a TrkC specific ligand. TrkC-GFP-NSCs had a significantly higher percentage of neuronal differentiation when treated with NT-3 relative to the rAd-LacZ control cells (55.2% vs. 29.8%; P<0.05, chi(2) test). Thus, our rAd-TrkC vector can transfect NSCs and produce functional TrkC receptors to promote neuronal differentiation of NSCs.

Similar articles

See all similar articles

Cited by 7 articles

See all "Cited by" articles

Publication types

MeSH terms

Feedback