Insertion of two pyrene moieties into oligodeoxyribonucleotides for the efficient detection of deletion polymorphisms

Chem Commun (Camb). 2006 Jul 14:(26):2768-70. doi: 10.1039/b604776a.

Abstract

For the detection of deletion polymorphisms, two pyrene moieties are tethered to an oligodeoxyribonucleotide (ODN) on both sides of the intervening base; one- and two-base deletions can be selectively detected by the strength of the excimer emission.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA Mutational Analysis / methods*
  • Gene Deletion*
  • Oligodeoxyribonucleotides / chemistry*
  • Oligodeoxyribonucleotides / genetics*
  • Polymorphism, Genetic / genetics*
  • Pyrenes / chemistry*

Substances

  • Oligodeoxyribonucleotides
  • Pyrenes
  • pyrene