Multicellular Rosette Formation Links Planar Cell Polarity to Tissue Morphogenesis

Dev Cell. 2006 Oct;11(4):459-70. doi: 10.1016/j.devcel.2006.09.007.

Abstract

Elongation of the body axis is accompanied by the assembly of a polarized cytoarchitecture that provides the basis for directional cell behavior. We find that planar polarity in the Drosophila embryo is established through a sequential enrichment of actin-myosin cables and adherens junction proteins in complementary surface domains. F-actin accumulation at AP interfaces represents the first break in planar symmetry and occurs independently of proper junctional protein distribution at DV interfaces. Polarized cells engage in a novel program of locally coordinated behavior to generate multicellular rosette structures that form and resolve in a directional fashion. Actin-myosin structures align across multiple cells during rosette formation, and adherens junction proteins assemble in a stepwise fashion during rosette resolution. Patterning genes essential for axis elongation selectively affect the frequency and directionality of rosette formation. We propose that the generation of higher-order rosette structures links local cell interactions to global tissue reorganization during morphogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / biosynthesis
  • Adherens Junctions
  • Alleles
  • Animals
  • Body Patterning
  • Cadherins / metabolism
  • Cell Movement
  • Cell Polarity*
  • Drosophila / cytology
  • Drosophila / embryology
  • Drosophila / genetics
  • Drosophila / metabolism
  • Drosophila Proteins / analysis
  • Drosophila Proteins / metabolism
  • Embryo, Nonmammalian / cytology
  • Embryo, Nonmammalian / embryology
  • Embryo, Nonmammalian / metabolism
  • Epithelial Cells / cytology
  • Epithelial Cells / metabolism
  • Green Fluorescent Proteins / metabolism
  • Immunohistochemistry
  • Morphogenesis*
  • Myosin Type II / biosynthesis

Substances

  • Actins
  • Cadherins
  • Drosophila Proteins
  • Green Fluorescent Proteins
  • Myosin Type II