Possible role of light in the maintenance of EIN3/EIL1 stability in Arabidopsis seedlings

Biochem Biophys Res Commun. 2006 Nov 17;350(2):484-91. doi: 10.1016/j.bbrc.2006.09.074. Epub 2006 Sep 25.


To examine the mechanism of EIN3-mediated gene expression by ethylene, the expression patterns of ethylene-inducible genes by ethylene were monitored in Col-0 and ethylene signaling mutants. In Col-0, the inducibility of ACC oxidase by ethylene in light-grown seedlings was much higher than in dark-grown seedlings. While the expression of ACC oxidase was highly increased by ethylene not only in Col-0 but in ein3-1 under light treatment, this pattern was completely abrogated in etiolated ein3-1 seedlings, suggesting the expression of EIN3-mediated ACC oxidase genes could be affected by light. To check if the level of EIN3 and EIL1 was regulated by light, cell-free degradation assays were performed. This resulted in the rapid degradation of these proteins within 1h after adding dark-grown cell extracts and this degradation was retarded by light-grown extracts. Here, we propose that light may act as a negative regulator in the destabilization of EIN3/EIL1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Oxidoreductases / biosynthesis
  • Amino Acid Oxidoreductases / genetics
  • Arabidopsis / genetics
  • Arabidopsis / growth & development
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / metabolism*
  • DNA-Binding Proteins
  • Darkness
  • Ethylenes / pharmacology
  • Gene Expression Regulation, Plant*
  • Light*
  • Mutation
  • Nuclear Proteins / metabolism*
  • Seedlings / genetics
  • Seedlings / metabolism
  • Signal Transduction / genetics
  • Transcription Factors / metabolism*


  • Arabidopsis Proteins
  • DNA-Binding Proteins
  • EIL1 protein, Arabidopsis
  • EIN3 protein, Arabidopsis
  • Ethylenes
  • Nuclear Proteins
  • Transcription Factors
  • ethylene
  • Amino Acid Oxidoreductases
  • 1-aminocyclopropane-1-carboxylic acid oxidase