Interaction of substance P, compound 48/80 and mastoparan with the alpha-subunit C-terminus of G protein

Immunol Lett. 1990 Sep;25(4):355-7. doi: 10.1016/0165-2478(90)90207-7.

Abstract

Pretreatment of purified calf brain G proteins with activated pertussis toxin or antibodies raised against the C-terminus of their alpha subunits prevented the increase in GTPase activity induced by substance P, compound 48/80 and mastoparan. These results suggest that these mast cell secretagogues activate G proteins directly via an interaction with the C-terminus of alpha subunits of G proteins by mimicking the agonist-liganded receptors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies / immunology
  • Cattle
  • Enzyme Activation / drug effects
  • GTP Phosphohydrolases / metabolism*
  • GTP-Binding Proteins / metabolism*
  • Intercellular Signaling Peptides and Proteins
  • Molecular Sequence Data
  • Peptide Fragments / immunology
  • Peptides
  • Pertussis Toxin
  • Protein Binding
  • Substance P / metabolism*
  • Virulence Factors, Bordetella / pharmacology
  • Wasp Venoms / pharmacology*
  • p-Methoxy-N-methylphenethylamine / pharmacology*

Substances

  • Antibodies
  • Intercellular Signaling Peptides and Proteins
  • Peptide Fragments
  • Peptides
  • Virulence Factors, Bordetella
  • Wasp Venoms
  • Substance P
  • p-Methoxy-N-methylphenethylamine
  • mastoparan
  • Pertussis Toxin
  • GTP Phosphohydrolases
  • GTP-Binding Proteins