Abstract
Pretreatment of purified calf brain G proteins with activated pertussis toxin or antibodies raised against the C-terminus of their alpha subunits prevented the increase in GTPase activity induced by substance P, compound 48/80 and mastoparan. These results suggest that these mast cell secretagogues activate G proteins directly via an interaction with the C-terminus of alpha subunits of G proteins by mimicking the agonist-liganded receptors.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies / immunology
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Cattle
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Enzyme Activation / drug effects
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GTP Phosphohydrolases / metabolism*
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GTP-Binding Proteins / metabolism*
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Intercellular Signaling Peptides and Proteins
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Molecular Sequence Data
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Peptide Fragments / immunology
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Peptides
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Pertussis Toxin
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Protein Binding
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Substance P / metabolism*
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Virulence Factors, Bordetella / pharmacology
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Wasp Venoms / pharmacology*
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p-Methoxy-N-methylphenethylamine / pharmacology*
Substances
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Antibodies
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Intercellular Signaling Peptides and Proteins
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Peptide Fragments
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Peptides
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Virulence Factors, Bordetella
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Wasp Venoms
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Substance P
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p-Methoxy-N-methylphenethylamine
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mastoparan
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Pertussis Toxin
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GTP Phosphohydrolases
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GTP-Binding Proteins