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. 2006 Dec;188(24):8655-7.
doi: 10.1128/JB.01049-06. Epub 2006 Sep 29.

Iron starvation triggers the stringent response and induces amino acid biosynthesis for bacillibactin production in Bacillus subtilis

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Iron starvation triggers the stringent response and induces amino acid biosynthesis for bacillibactin production in Bacillus subtilis

Marcus Miethke et al. J Bacteriol. 2006 Dec.

Abstract

Iron deprivation in bacteria causes the derepression of genes controlled by the ferric uptake regulator (Fur). The present microarray analysis of iron-starved Bacillus subtilis cells grown in minimal medium unveils additional physiological effects on a large number of genes linked to stringent-response regulation and to genes involved in amino acid biosynthesis associated with pathways essential for bacillibactin production.

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Figures

FIG. 1.
FIG. 1.
Dot blot analysis of selected genes. Shown are the relative transcriptional levels of cells cultured in minimal medium under iron-starved (−) or iron-replete (+) conditions. Panels: A, Fur-regulated genes (as controls); B, amino acid biosynthesis genes of the threonine (yclM, hom), serine/glycine (yoaD), and glutamate (gltA) pathways; C, stringent-response-regulated genes; D, CodY-regulated genes; E, genes of the tricarboxylic acid cycle. The arrows indicate increased (↑), decreased (↓), or equal (→) transcript amounts that were detected during iron starvation in comparison with iron-replete conditions.
FIG. 2.
FIG. 2.
Scheme of bacillibactin synthesis. The bacillibactin precursors glycine and threonine derive from the primary metabolism. The synthesis of 2,3-dihydroxybenzoate (DHB) and the nonribosomally catalyzed bacillibactin assembly are Fur regulated in the secondary metabolism. The numbers shown indicate the numbers of genes that were upregulated during iron starvation. The total numbers of activities involved in the presented pathway sections are in parentheses. reg., regulation.

References

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