Yonghaparkia alkaliphila gen. nov., sp. nov., a novel member of the family Microbacteriaceae isolated from an alkaline soil

Int J Syst Evol Microbiol. 2006 Oct;56(Pt 10):2415-2420. doi: 10.1099/ijs.0.64258-0.

Abstract

Two Gram-positive, non-motile, non-spore-forming and rod-shaped actinomycete strains, KSL-113(T) and KSL-133, were isolated from an alkaline soil in Korea, and their taxonomic positions were investigated by using a polyphasic approach. The strains grew optimally at 30 degrees C and pH 9.0. Phenotypic, phylogenetic and genetic similarities indicated that strains KSL-113(T) and KSL-133 represent the same species. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains KSL-113(T) and KSL-133 fell within the family Microbacteriaceae of the suborder Micrococcineae, the highest 16S rRNA gene sequence similarity values (98.2 %) being obtained with respect to Microcella putealis CV-2(T). The 16S rRNA gene sequence similarities between strains KSL-113(T) and KSL-133 and the other members of the family Microbacteriaceae used in the phylogenetic analysis were less than 96.0 %. Strains KSL-113(T) and KSL-133 could be clearly distinguished from members of the family Microbacteriaceae on the basis of differences in chemotaxonomic properties, including the predominant menaquinone type, the cell-wall peptidoglycan type and the fatty acid profile. Accordingly, on the basis of the combined phenotypic, chemotaxonomic and phylogenetic data, strains KSL-113(T) and KSL-133 constitute a novel genus and species of the family Microbacteriaceae, for which the name Yonghaparkia alkaliphila gen. nov., sp. nov. is proposed. The type strain of Yonghaparkia alkaliphila is KSL-113(T) (=KCTC 19126(T)=CIP 108920(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinomycetales / chemistry
  • Actinomycetales / classification*
  • Actinomycetales / genetics
  • Actinomycetales / isolation & purification
  • Bacterial Typing Techniques
  • DNA, Bacterial / analysis
  • DNA, Ribosomal
  • Fatty Acids / analysis
  • Genes, rRNA
  • Genotype
  • Hydrogen-Ion Concentration
  • Molecular Sequence Data
  • Phenotype
  • Phylogeny
  • RNA, Ribosomal, 16S / analysis
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Soil Microbiology*

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/DQ256087
  • GENBANK/DQ256088