Interleukin 4 down-regulates the expression of CD14 in normal human monocytes

Eur J Immunol. 1990 Nov;20(11):2375-81. doi: 10.1002/eji.1830201103.


Recombinant interleukin 4 (IL4) down-regulates the expression of CD14 on normal human monocytes, as assessed by flow cytometry, binding assays with radiolabeled anti-CD14 monoclonal antibody (mAb), and immunoprecipitation of 125I-labeled monocytes with anti-CD14 mAb. In parallel, CD23 expression on monocytes was strongly increased by IL4 stimulation, as assessed by both flow cytometry and immunoprecipitation. Down-regulation of surface CD14 was first detectable after 24-36 h of incubation with rIL4, and was almost complete after 4 days of culture. None of the other recombinant lymphokines tested (IL1, IL2, IL3, IL5, IL6, interferon-gamma, tumor necrosis factor alpha and beta, granulocyte-macrophage colony-stimulating factor) decreased CD14 expression. Metabolic labeling studies with [35S]methionine showed that both the membrane-associated and the soluble form of CD14 are decreased by IL4 stimulation. Northern blot analysis showed that incubation of monocytes with IL4 induced a marked decrease in CD14 mRNA. Nuclear run-off assays revealed that the IL4-dependent down-regulation of CD14 resulted from decreased transcription. Thus, IL4 exerts specific and opposite effects on the expression of monocytic antigens.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antigens, CD / analysis*
  • Antigens, Differentiation, B-Lymphocyte / analysis
  • Antigens, Differentiation, Myelomonocytic / analysis*
  • Antigens, Differentiation, Myelomonocytic / genetics
  • Down-Regulation
  • HLA-DR Antigens / analysis
  • Humans
  • Interleukin-4 / pharmacology*
  • Interleukin-6 / pharmacology
  • Lipopolysaccharide Receptors
  • Monocytes / immunology*
  • Precipitin Tests
  • RNA, Messenger / analysis
  • Receptors, Fc / analysis
  • Receptors, IgE


  • Antigens, CD
  • Antigens, Differentiation, B-Lymphocyte
  • Antigens, Differentiation, Myelomonocytic
  • HLA-DR Antigens
  • Interleukin-6
  • Lipopolysaccharide Receptors
  • RNA, Messenger
  • Receptors, Fc
  • Receptors, IgE
  • Interleukin-4