Structure-function relationships among highly diverse T cells that recognize a determinant from influenza virus hemagglutinin

J Exp Med. 1990 Dec 1;172(6):1643-51. doi: 10.1084/jem.172.6.1643.

Abstract

We have analyzed the structural and genetic basis for T cell recognition of the complex formed between antigen and class II products of the major histocompatibility complex by performing sequence analysis of T cell receptors (TCRs) induced in response to the helper T cell site 1 of the influenza virus hemagglutinin. The results demonstrate, first, that structurally highly diverse TCRs can be utilized in recognition of the same antigen/I-Ed complex: 12 of 13 TCRs utilize unique V alpha/V beta gene segment combinations, suggesting that approximately 70 different V alpha/V beta combinations are available to BALB/c mice in response to this determinant. Second, comparison of these sequences with the ability of each hybridoma to recognize a panel of peptide analogues suggests that alpha and beta chains of these TCRs frequently determine specificity for the NH2-terminal and the COOH terminal portions, respectively, of the site 1 determinant.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Epitopes / immunology
  • Genes, MHC Class II
  • Hemagglutinin Glycoproteins, Influenza Virus
  • Hemagglutinins, Viral / immunology*
  • Macromolecular Substances
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Oligonucleotide Probes
  • Receptors, Antigen, T-Cell / genetics*
  • Receptors, Antigen, T-Cell / immunology
  • Sequence Homology, Nucleic Acid
  • T-Lymphocytes / immunology*
  • T-Lymphocytes, Helper-Inducer / immunology*

Substances

  • Epitopes
  • Hemagglutinin Glycoproteins, Influenza Virus
  • Hemagglutinins, Viral
  • Macromolecular Substances
  • Oligonucleotide Probes
  • Receptors, Antigen, T-Cell

Associated data

  • GENBANK/M34194
  • GENBANK/M34219