Identification of Borrelia burgdorferi ribosomal protein L25 by the phage surface display method and evaluation of the protein's value for serodiagnosis

J Clin Microbiol. 2006 Oct;44(10):3778-80. doi: 10.1128/JCM.00371-06.

Abstract

The phage surface display technique was used to identify Borrelia burgdorferi antigens. By affinity selection with immunoglobulin G from pooled sera of six Lyme borreliosis (LB) patients, the ribosomal protein L25 was identified. The diagnostic value of L25 was investigated by an enzyme-linked immunosorbent assay, using sera from 80 LB patients and 75 controls, and the use of the protein resulted in a specificity of 99% and a 23% sensitivity, which qualify L25 as a useful antigen when combined with others.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Borrelia burgdorferi / genetics*
  • Humans
  • Lyme Disease / diagnosis*
  • Middle Aged
  • Peptide Library*
  • Ribosomal Proteins / genetics*
  • Sensitivity and Specificity
  • Serologic Tests*

Substances

  • Peptide Library
  • Ribosomal Proteins
  • ribosomal protein L25