Abstract
CD40 is a 45- to 50-kilodalton transmembrane glycoprotein expressed on B lymphocytes, epithelial cells, and some carcinoma cell lines. Human resting B lymphocytes entered a state of sustained proliferation when incubated with both the mouse fibroblastic Ltk- cell line that had been transfected with the human Fc receptor (Fc gamma RII/CDw32) and monoclonal antibodies to CD40. In combination with interleukin-4, factor-dependent long-term normal human B cell lines were generated that were consistently negative for Epstein-Barr viral infection. Thus, cross-linking of CD40 is likely to represent an important phenomenon in the clonal expansion of B cells.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Antibodies, Monoclonal / pharmacology*
-
Antigens, CD / immunology
-
Antigens, CD / physiology
-
Antigens, Differentiation, B-Lymphocyte / immunology
-
Antigens, Differentiation, B-Lymphocyte / physiology*
-
B-Lymphocytes / cytology
-
B-Lymphocytes / immunology*
-
B-Lymphocytes / microbiology
-
CD40 Antigens
-
Cell Division
-
Fibroblasts / metabolism
-
Herpesvirus 4, Human / growth & development
-
Humans
-
Interleukin-4 / pharmacology*
-
Lymphocyte Activation
-
Receptors, Fc / genetics
-
Receptors, Fc / physiology
-
Recombinant Proteins / pharmacology
-
Transfection
Substances
-
Antibodies, Monoclonal
-
Antigens, CD
-
Antigens, Differentiation, B-Lymphocyte
-
CD40 Antigens
-
Receptors, Fc
-
Recombinant Proteins
-
Interleukin-4