CD27 expression by a distinct subpopulation of human B lymphocytes

Eur J Immunol. 1990 Dec;20(12):2679-84. doi: 10.1002/eji.1830201223.

Abstract

CD27 is present on the surface of a major subset of peripheral blood T lymphocytes. In this report we show that CD27 is also expressed on a subpopulation of the normal human B cell lineage which is absent from cord blood but present in tonsils and in the peripheral blood of adult individuals. CD27+ B lymphocytes are characterized by the following criteria: (a) in terms of physical properties, the CD27+ B cells form a population with an increased cell size combined with a decreased cell density; (b) the CD27 expression of tonsillar B lymphocytes is postively correlated with mIgA but negatively correlated with membrane IgM/membrane IgD positivity; (c) CD27 on B cells can be induced selectively by the combination of Staphylococcus aureus plus interleukin 2, but not by either treatment alone, and (d) CD27+ B lymphocytes express high levels of the adhesion structures LFA-1 (CD11a), ICAM-1 (CD54), LFA-3 (CD58) and of the lymphocyte homing receptor CD44. These latter findings suggest that CD27+ B cells are predispose to form cell-cell interactions. Accordingly, within 3 h of cell culture CD27+, but not CD27-, B lymphocytes were found to form LFA-1-mediated homotypic B cell clusters.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal
  • Antigens, CD / metabolism*
  • Antigens, Differentiation / metabolism
  • Antigens, Differentiation, B-Lymphocyte / metabolism*
  • Antigens, Differentiation, T-Lymphocyte / metabolism*
  • B-Lymphocyte Subsets / cytology
  • B-Lymphocyte Subsets / immunology*
  • CD5 Antigens
  • Cell Adhesion Molecules / metabolism
  • Cell Aggregation
  • Cell Differentiation
  • Cells, Cultured
  • Flow Cytometry
  • Humans
  • Immunoglobulin D / metabolism
  • In Vitro Techniques
  • Lymphocyte Activation
  • Palatine Tonsil / cytology
  • Receptors, Antigen, B-Cell / metabolism
  • Tumor Necrosis Factor Receptor Superfamily, Member 7

Substances

  • Antibodies, Monoclonal
  • Antigens, CD
  • Antigens, Differentiation
  • Antigens, Differentiation, B-Lymphocyte
  • Antigens, Differentiation, T-Lymphocyte
  • CD5 Antigens
  • Cell Adhesion Molecules
  • Immunoglobulin D
  • Receptors, Antigen, B-Cell
  • Tumor Necrosis Factor Receptor Superfamily, Member 7