CD8alpha+ dendritic cells are required for efficient entry of Listeria monocytogenes into the spleen

Immunity. 2006 Oct;25(4):619-30. doi: 10.1016/j.immuni.2006.07.017. Epub 2006 Oct 5.


In addition to their bridging function between innate and adaptive immunity, dendritic cells (DCs) may also contribute to primary resistance against infection. Here we analyzed the role of DCs during infection with Listeria monocytogenes by performing systemic in vivo depletion of these cells. We showed that CD8alpha(+) DCs were crucial for L. monocytogenes spreading and proliferation in the spleen. Efficient and rapid uptake of L. monocytogenes by CD8alpha(+) DCs required the small GTPase Rac1 and is a general characteristic of this DC subpopulation in filtering particles out of the blood. Thus, CD8alpha(+) DCs appear to play an important role for efficient bacterial entry into the spleen, which is of relevance for subsequent immune responses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CD11c Antigen / analysis
  • CD8 Antigens / analysis*
  • Dendritic Cells / immunology*
  • Dendritic Cells / microbiology
  • Dendritic Cells / transplantation
  • Granulocytes / immunology
  • Listeria monocytogenes*
  • Listeriosis / enzymology
  • Listeriosis / immunology*
  • Lymphocyte Activation
  • Mice
  • Mice, Inbred Strains
  • Neuropeptides / genetics
  • Neuropeptides / metabolism
  • Spleen / cytology
  • Spleen / immunology
  • Spleen / microbiology*
  • T-Lymphocytes / immunology
  • rac GTP-Binding Proteins / genetics
  • rac GTP-Binding Proteins / metabolism
  • rac1 GTP-Binding Protein


  • CD11c Antigen
  • CD8 Antigens
  • CD8alpha antigen
  • Neuropeptides
  • Rac1 protein, mouse
  • rac GTP-Binding Proteins
  • rac1 GTP-Binding Protein