Cloning and characterization of a novel small monomeric GTPase, RasL10B, with tumor suppressor potential

Biotechnol Lett. 2006 Dec;28(23):1901-8. doi: 10.1007/s10529-006-9176-6. Epub 2006 Sep 23.

Abstract

Ras proteins are members of the superfamily of small GTPase. A novel human Ras-like transcript, termed RasL10B, was isolated from human blood cell cDNA library. RasL10B gene contains four exons and three introns, which encodes a 203 amino acid protein with a molecular mass of about 23.2 kDa. RT-PCR analysis showed that RasL10B is expressed extensively in human tissues. Subcellular location analysis of GFP-RasL10B fusion protein revealed that RasL10B was distributed to the cytoplasm of COS7 cells. In addition, RasL10B was expressed in E. coli Rosette (DE3) and purified to a homogenicity by Ni-NTA affinity chromatography. Finally, the mRNA levels of RasL10B were down-regulated in all human breast cancer cell lines we tested. In summary, RasL10B is a new member of Ras superfamily with tumor suppressor potential.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • COS Cells
  • Cell Line, Tumor
  • Chlorocebus aethiops
  • Cloning, Molecular
  • Cytoplasm / metabolism
  • Escherichia coli / metabolism
  • GTP Phosphohydrolases / chemistry*
  • Genes, Tumor Suppressor*
  • Humans
  • Molecular Sequence Data
  • Sequence Homology, Amino Acid
  • Tissue Distribution
  • ras Proteins / chemistry*
  • ras Proteins / genetics*

Substances

  • RASL10B protein, human
  • GTP Phosphohydrolases
  • ras Proteins