Abstract
The defatted seed meal of common vetch (Vicia sativa L.) served as a source of (R)-oxynitrilase which catalyzed the enantioselective addition of HCN to aromatic, heteroaromatic, fluoro-substituted aromatic aldehydes to produce the corresponding enantiomeric pure cyanohydrins in yields of 52-100% and 88-99% ee at 12 degrees C under micro-aqueous medium (diisopropyl ether) without addition of buffer solution.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aldehyde-Lyases / chemical synthesis
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Aldehyde-Lyases / chemistry*
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Biotechnology / methods
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Biotransformation
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Magnetic Resonance Spectroscopy
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Models, Chemical
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Nitriles / chemical synthesis
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Nitriles / chemistry*
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Plant Extracts / chemistry*
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Plants / metabolism
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Stereoisomerism
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Temperature
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Vicia sativa / enzymology*
Substances
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Nitriles
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Plant Extracts
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cyanohydrin
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Aldehyde-Lyases
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mandelonitrile lyase