Accumulation of chloroplast DNA sequences on the Y chromosome of Silene latifolia

Genetica. Sep-Nov 2006;128(1-3):167-75. doi: 10.1007/s10709-005-5701-0.

Abstract

Silene latifolia is a model dioecious plant with heteromorphic sex chromosomes. The Y chromosome is the largest in this species. Theoretical models propose an accumulation of repetitive DNA sequences in non-recombining parts of the Y chromosome. In this study, we isolated a BAC7H5 clone preferentially hybridizing to the Y chromosome of S. latifolia. Sequence analysis revealed that this BAC7H5 contains part of the chloroplast genome, indicating that these chloroplast sequences have accumulated on the Y chromosome and also may contribute to its large size. We constructed Y chromosome- and X chromosome-specific libraries and screened them to find Y- and/or X-linked copies of chloroplast sequences. Sequence analysis revealed higher divergence of a non-genic region of the chloroplast sequences located on the Y chromosome while genic regions tested showed only very low (max 0.9%) divergence from their chloroplast homologues.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Chromosomes, Artificial, Bacterial / genetics
  • Chromosomes, Plant / genetics*
  • DNA Primers / genetics
  • DNA, Chloroplast / genetics*
  • Evolution, Molecular
  • In Situ Hybridization, Fluorescence
  • Models, Genetic
  • Molecular Sequence Data
  • Sequence Homology, Nucleic Acid
  • Silene / genetics*

Substances

  • DNA Primers
  • DNA, Chloroplast