Association of B cell antigen receptor with protein tyrosine kinase Lyn

Science. 1991 Jan 11;251(4990):192-4. doi: 10.1126/science.1702903.


Antigen is thought to cross-link membrane-bound immunoglobulins (Igs) of B cells, causing proliferation and differentiation or the inhibition of growth. Protein tyrosine kinases are probably involved in signal transduction for cell proliferation and differentiation. The Src-like protein tyrosine kinase Lyn is expressed preferentially in B cells. The Lyn protein and its kinase activity could be coimmunoprecipitated with IgM from detergent lysates. Cross-linking of membrane-bound IgM induced a rapid increase in tyrosine phosphorylation of at least ten distinct proteins of B cells. Thus, Lyn is physically associated with membrane-bound IgM, and is suggested to participate in antigen-mediated signal transduction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • B-Lymphocytes / immunology
  • Cell Line
  • Detergents
  • Electrophoresis, Polyacrylamide Gel
  • Immunoblotting
  • Immunoglobulin M / metabolism
  • Immunosorbent Techniques
  • Mice
  • Molecular Weight
  • Phosphoproteins / metabolism
  • Phosphorylation
  • Phosphotyrosine
  • Protein-Tyrosine Kinases / genetics
  • Protein-Tyrosine Kinases / metabolism*
  • Receptors, Antigen, B-Cell / metabolism*
  • Signal Transduction
  • Tyrosine / analogs & derivatives
  • Tyrosine / metabolism
  • src-Family Kinases*


  • Detergents
  • Immunoglobulin M
  • Phosphoproteins
  • Receptors, Antigen, B-Cell
  • Phosphotyrosine
  • Tyrosine
  • Protein-Tyrosine Kinases
  • lyn protein-tyrosine kinase
  • src-Family Kinases