Semiquantitative one-step RT-PCR for simultaneous identification of human influenza and respiratory syncytial viruses

X Sherry Chi; Fenglan Li; John S Tam; Ruth Rappaport; Sheau-Mei Cheng

doi:10.1016/j.jviromet.2006.08.015

Semiquantitative one-step RT-PCR for simultaneous identification of human influenza and respiratory syncytial viruses

J Virol Methods. 2007 Jan;139(1):90-2. doi: 10.1016/j.jviromet.2006.08.015. Epub 2006 Oct 9.

Authors

X Sherry Chi¹, Fenglan Li, John S Tam, Ruth Rappaport, Sheau-Mei Cheng

Affiliation

¹ Department of Clinical Immunology and Virology, Applied Immunology and Microbiology Division, Wyeth Vaccines Research, Bldg 180, 401N. Middletown Rd., Pearl River, NY 10965, USA.

Abstract

A multiplex reverse transcription-polymerase chain reaction (mRT-PCR) method was developed for simultaneous detection and typing/subtyping of influenza viruses A/H1, A/H3 or B, and respiratory syncytial viruses A or B, followed by DNA semiquantitation using the Agilent 2100 Bioanalyzer. Such method provides a rapid, specific and sensitive diagnostic tool for detection and semiquantification of respiratory illness specimens.

MeSH terms

Child, Preschool
Humans
Infant
Influenza A virus / isolation & purification*
Influenza B virus / isolation & purification*
Respiratory Syncytial Viruses / isolation & purification*
Reverse Transcriptase Polymerase Chain Reaction / methods*
Sensitivity and Specificity