Alpha,beta-dicarbonyl reduction by Saccharomyces D-arabinose dehydrogenase

Biochim Biophys Acta. 2006 Nov;1760(11):1636-45. doi: 10.1016/j.bbagen.2006.08.025. Epub 2006 Sep 8.


An alpha,beta-dicarbonyl reductase activity was purified from Saccharomyces cerevisiae and identified as the cytosolic enzyme D-Arabinose dehydrogenase (ARA1) by MALDI-TOF/TOF. Size exclusion chromatography analysis of recombinant Ara1p revealed that this protein formed a homodimer. Ara1p catalyzed the reduction of the reactive alpha,beta-dicarbonyl compounds methylglyoxal, diacetyl, and pentanedione in a NADPH dependant manner. Ara1p had apparent Km values of approximately 14 mM, 7 mM and 4 mM for methylglyoxal, diacetyl and pentanedione respectively, with corresponding turnover rates of 4.4, 6.9 and 5.9 s(-1) at pH 7.0. pH profiling showed that Ara1p had a pH optimum of 4.5 for the diacetyl reduction reaction. Ara1p also catalyzed the NADP+ dependant oxidation of acetoin; however this back reaction only occurred at alkaline pH values. That Ara1p was important for degradation of alpha,beta-dicarbonyl substrates was further supported by the observation that ara1-Delta knockout yeast mutants exhibited a decreased growth rate phenotype in media containing diacetyl.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetoin Dehydrogenase / chemistry
  • Acetoin Dehydrogenase / isolation & purification
  • Amino Acid Sequence
  • Diacetyl / chemistry
  • Diacetyl / metabolism
  • Kinetics
  • Mass Spectrometry
  • Molecular Sequence Data
  • Oxidation-Reduction
  • Oxidoreductases Acting on Aldehyde or Oxo Group Donors / chemistry*
  • Oxidoreductases Acting on Aldehyde or Oxo Group Donors / genetics
  • Oxidoreductases Acting on Aldehyde or Oxo Group Donors / metabolism
  • Pyruvaldehyde / chemistry
  • Pyruvaldehyde / metabolism
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins / chemistry*
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism
  • Sequence Alignment
  • Sugar Alcohol Dehydrogenases / chemistry*
  • Sugar Alcohol Dehydrogenases / genetics
  • Sugar Alcohol Dehydrogenases / metabolism


  • Ara1 protein, S cerevisiae
  • Recombinant Proteins
  • Saccharomyces cerevisiae Proteins
  • Pyruvaldehyde
  • Sugar Alcohol Dehydrogenases
  • Acetoin Dehydrogenase
  • D-arabinose dehydrogenase
  • Oxidoreductases Acting on Aldehyde or Oxo Group Donors
  • Diacetyl