Signature-tagged transposon mutagenesis identifies novel Mycobacterium tuberculosis genes involved in the parasitism of human macrophages

Infect Immun. 2007 Jan;75(1):504-7. doi: 10.1128/IAI.00058-06. Epub 2006 Oct 9.


Using signature-tagged transposon mutagenesis, we isolated 23 Mycobacterium tuberculosis mutants, corresponding to 21 genes or genetic regions, attenuated in their ability to parasitize human macrophages. Mutants disrupted in the ABC transporter-encoding genes Rv0986 and Rv0987 were further characterized as being impaired in their ability to bind to host cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Proteins / metabolism
  • DNA Transposable Elements / genetics*
  • Female
  • Genes, Bacterial*
  • Humans
  • Macrophages / parasitology*
  • Mice
  • Mice, Inbred C57BL
  • Mutagenesis
  • Mycobacterium tuberculosis / genetics*
  • Mycobacterium tuberculosis / pathogenicity*


  • Bacterial Proteins
  • DNA Transposable Elements