Collagen-platelet rich plasma hydrogel enhances primary repair of the porcine anterior cruciate ligament

J Orthop Res. 2007 Jan;25(1):81-91. doi: 10.1002/jor.20282.


The anterior cruciate ligament (ACL) fails to heal after suture repair. One hypothesis for this failure is the premature loss of the fibrin clot, or provisional scaffolding, between the two ligament ends in the joint environment. To test this hypothesis, a substitute provisional scaffold of collagen-platelet rich plasma (PRP) hydrogel was used to fill the ACL wound site at the time of suture repair and the structural properties of the healing ACLs evaluated 4 weeks after surgery. Bilateral ACL transections were performed in five 30-kg Yorkshire pigs and treated with suture repair. In each animal, one of the repairs was augmented with placement of a collagen-PRP hydrogel at the ACL transection site, while the contralateral knee had suture repair alone. In addition, six control knees with intact ACLs from three additional animals were used as a control group. No postoperative immobilization was used. After 4 weeks the animals underwent in vivo magnetic resonance imaging to assess the size of the healing ACL, followed by biomechanical testing to determine tensile properties. The supplementation of suture repair with a collagen-PRP hydrogel resulted in significant improvements in load at yield, maximum load, and linear stiffness at 4 weeks. We conclude that use of a stabilized provisional scaffold, such as a collagen-PRP hydrogel, to supplement primary repair of the ACL can result in improved biomechanical properties at an early time point. Further studies to determine the long-term effect of primary repair enhancement are needed.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Anterior Cruciate Ligament Injuries*
  • Biomechanical Phenomena
  • Cicatrix / pathology
  • Collagen / blood*
  • Collagen / pharmacology
  • Female
  • Hindlimb / injuries
  • Hydrogels / pharmacology*
  • Knee Injuries / therapy
  • Magnetic Resonance Imaging
  • Platelet-Rich Plasma / metabolism*
  • Suture Techniques*
  • Swine
  • Wound Healing / drug effects*


  • Hydrogels
  • Collagen