A simple and rapid method for the detection of RNA in formalin-fixed, paraffin-embedded tissues by PCR amplification

Biochem Biophys Res Commun. 1991 Jan 15;174(1):176-80. doi: 10.1016/0006-291x(91)90502-x.

Abstract

A simple and rapid method for the extraction, reverse transcription and PCR amplification of RNA from formalin-fixed, paraffin-embedded tissue is described. The procedure can be completed within 24 hours. In a first application of this method we detect human albumin mRNA in liver tissue, demonstrating the feasibility to retrospectively analyze gene expression and RNA viruses in fixed tissue.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Albumins / genetics
  • Biopsy, Needle
  • DNA / biosynthesis
  • Fixatives*
  • Formaldehyde*
  • Gene Expression
  • Humans
  • Introns
  • Liver / chemistry*
  • Paraffin*
  • Polymerase Chain Reaction
  • RNA Splicing
  • RNA Viruses / genetics
  • RNA, Messenger / analysis*
  • RNA-Directed DNA Polymerase / genetics
  • RNA-Directed DNA Polymerase / isolation & purification
  • Sensitivity and Specificity

Substances

  • Albumins
  • Fixatives
  • RNA, Messenger
  • Formaldehyde
  • Paraffin
  • DNA
  • RNA-Directed DNA Polymerase