A group M consensus envelope glycoprotein induces antibodies that neutralize subsets of subtype B and C HIV-1 primary viruses

Virology. 2006 Sep 30;353(2):268-82. doi: 10.1016/j.virol.2006.04.043.


HIV-1 subtype C is the most common HIV-1 group M subtype in Africa and many parts of Asia. However, to date HIV-1 vaccine candidate immunogens have not induced potent and broadly neutralizing antibodies against subtype C primary isolates. We have used a centralized gene strategy to address HIV-1 diversity and generated a group M consensus envelope gene with shortened consensus variable loops (CON-S) for comparative studies with wild-type (WT) Env immunogens. Our results indicate that the consensus HIV-1 group M CON-S Env elicited cross-subtype neutralizing antibodies of similar or greater breadth and titer than the WT Envs tested, indicating the utility of a centralized gene strategy. Our study also shows the feasibility of iterative improvements in Env immunogenicity by rational design of centralized genes.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • AIDS Vaccines / immunology
  • Amino Acid Sequence
  • Animals
  • Animals, Outbred Strains
  • Consensus Sequence
  • Cross Reactions
  • Gene Products, env / chemical synthesis
  • Gene Products, env / genetics
  • Gene Products, env / immunology*
  • Guinea Pigs
  • HIV Antibodies / immunology*
  • HIV Infections / immunology*
  • HIV-1 / classification
  • HIV-1 / immunology*
  • Immunophenotyping
  • Molecular Sequence Data
  • Neutralization Tests
  • env Gene Products, Human Immunodeficiency Virus


  • AIDS Vaccines
  • Gene Products, env
  • HIV Antibodies
  • env Gene Products, Human Immunodeficiency Virus
  • gp140 envelope protein, Human immunodeficiency virus 1