Developmentally controlled farnesylation modulates AtNAP1;1 function in cell proliferation and cell expansion during Arabidopsis leaf development

Plant Physiol. 2006 Dec;142(4):1412-26. doi: 10.1104/pp.106.088344. Epub 2006 Oct 13.


In multicellular organisms, organogenesis requires tight control and coordination of cell proliferation, cell expansion, and cell differentiation. We have identified Arabidopsis (Arabidopsis thaliana) nucleosome assembly protein 1 (AtNAP1;1) as a component of a regulatory mechanism that connects cell proliferation to cell growth and expansion during Arabidopsis leaf development. Molecular, biochemical, and kinetic studies of AtNAP1;1 gain- or loss-of-function mutants indicate that AtNAP1;1 promotes cell proliferation or cell expansion in a developmental context and as a function of the farnesylation status of the protein. AtNAP1;1 was farnesylated and localized to the nucleus during the cell proliferation phase of leaf development when it promotes cell division. Later in leaf development, nonfarnesylated AtNAP1;1 accumulates in the cytoplasm when it promotes cell expansion. Ectopic expression of nonfarnesylated AtNAP1;1, which localized to the cytoplasm, disrupts this developmental program by promoting unscheduled cell expansion during the proliferation phase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / analysis
  • Adenosine Triphosphatases / genetics
  • Adenosine Triphosphatases / metabolism*
  • Arabidopsis / cytology
  • Arabidopsis / growth & development*
  • Arabidopsis / metabolism
  • Arabidopsis Proteins / analysis
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Cell Differentiation
  • Cell Enlargement*
  • Cell Proliferation*
  • Gene Expression Regulation, Plant
  • Green Fluorescent Proteins / analysis
  • Mutation
  • Plant Leaves / cytology
  • Plant Leaves / growth & development
  • Plant Leaves / metabolism
  • Protein Prenylation / physiology*
  • Recombinant Fusion Proteins / analysis


  • Arabidopsis Proteins
  • Recombinant Fusion Proteins
  • Green Fluorescent Proteins
  • Adenosine Triphosphatases
  • NAP1 protein, Arabidopsis