A DNA chip-based spoligotyping method for the strain identification of Mycobacterium tuberculosis isolates

Eun Ju Song; Hae Jin Jeong; Sun Min Lee; Cheol Min Kim; Eun Sil Song; Young Kil Park; Gill-Han Bai; Eun Yup Lee; Chulhun L Chang

doi:10.1016/j.mimet.2006.09.005

A DNA chip-based spoligotyping method for the strain identification of Mycobacterium tuberculosis isolates

J Microbiol Methods. 2007 Feb;68(2):430-3. doi: 10.1016/j.mimet.2006.09.005. Epub 2006 Oct 12.

Authors

Eun Ju Song¹, Hae Jin Jeong, Sun Min Lee, Cheol Min Kim, Eun Sil Song, Young Kil Park, Gill-Han Bai, Eun Yup Lee, Chulhun L Chang

Affiliation

¹ Department of Laboratory Medicine, School of Medicine, Pusan National University, Seo-Gu Ami-Dong 1-10, Busan 602-739, Republic of Korea.

PMID: 17046089
DOI: 10.1016/j.mimet.2006.09.005

Abstract

The clinical efficacy of a spoligotyping method for discriminating Mycobacterium tuberculosis strains was evaluated. Among the strains other than Beijing or Beijing like family, 30 different spoligotypes out of 39 strains were produced, which included 4 strains not having IS6110 sequence. The oligonucleotide chip-based spoligotyping technique is useful for early screening and detection of clonal proximity of M. tuberculosis isolates.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA Fingerprinting / methods*
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Intergenic / chemistry
DNA, Intergenic / genetics
Humans
Mycobacterium tuberculosis / classification*
Mycobacterium tuberculosis / genetics
Mycobacterium tuberculosis / isolation & purification
Oligonucleotide Array Sequence Analysis / methods*
Tuberculosis / microbiology*

Substances

DNA, Bacterial
DNA, Intergenic