Bacterial biofilm formation is the prevailing microbial lifestyle in natural and manmade environments and occurs on all surface types. Biofilm formation develops in several phases and is influenced by various parameters, both environmental and inherent to the attaching cell. Biofilms also serve as protective niches for particular pathogens when outside a host. Although it is accepted that biofilms are ubiquitous in nature, the significance of biofilms in clinical settings, especially with regard to their role in medical-related infections, is often underestimated. It has been found that several aspects of human pathogenesis within a clinical context are directly related to biofilm development. Various types of surfaces in clinical settings are prone to biofilm development and an increased risk of disease may be a direct consequence of their formation. This review describes the process of biofilm formation, highlights the importance of bacterial associations with surfaces in clinical settings and describes various methods for biofilm visualization and control.