Granulocyte colony-stimulating factor enhances the phagocytic and bactericidal activity of normal and defective human neutrophils

J Infect Dis. 1991 Mar;163(3):579-83. doi: 10.1093/infdis/163.3.579.


Granulocyte colony-stimulating factor (G-CSF) stimulates proliferation of myeloid cells and may be a valuable adjunct in prevention and treatment of neutropenia-associated infections. Neutrophil (PMNL) phagocytic and microbicidal functions against Staphylococcus aureus and Candida albicans blastoconidia were therefore evaluated. Bacterial phagocytosis and bactericidal activity were significantly enhanced by approximately 50%-70% after preincubation of normal PMNL with G-CSF in concentrations of 1000-4000 units/ml for 10 min at 37 degrees C. G-CSF in similar concentrations enhanced the defective bactericidal activity of PMNL from HIV-1-infected patients by approximately 70%-150% and reached the baseline control PMNL killing. However, G-CSF enhanced neither phagocytosis nor fungicidal activity of normal PMNL against C. albicans blastoconidia. These data demonstrate that G-CSF enhances the antibacterial but not the antifungal activities of human PMNL in vitro and also improves the defective PMNL bactericidal activity of HIV-1-infected patients.

MeSH terms

  • AIDS-Related Complex / immunology
  • Acquired Immunodeficiency Syndrome / immunology*
  • Adult
  • Analysis of Variance
  • Blood Bactericidal Activity / physiology*
  • Candida albicans / immunology
  • Child
  • Granulocyte Colony-Stimulating Factor / physiology*
  • Humans
  • In Vitro Techniques
  • Neutrophils / physiology*
  • Phagocytosis / physiology*
  • Reference Values
  • Staphylococcus aureus / immunology


  • Granulocyte Colony-Stimulating Factor