A mouse model of sensorimotor controlled cortical impact: characterization using longitudinal magnetic resonance imaging, behavioral assessments and histology

J Neurosci Methods. 2007 Mar 15;160(2):187-96. doi: 10.1016/j.jneumeth.2006.09.007. Epub 2006 Oct 16.

Abstract

The present study establishes a new mouse model for traumatic brain injury (TBI), using an electromechanically driven linear motor impactor device to deliver a lateral controlled cortical impact (CCI) injury to the sensorimotor cortex. Lesion cavity size was measured, and inter-animal consistency demonstrated, at 14 days post injury. Qualitative information regarding damage progression over time was obtained by scanning with high field magnetic resonance imaging (MRI) at five time points following injury. Functional impairment and recovery were measured with the Rotarod, gridwalk and cylinder tests, and lesion cavity volume was measured post mortem with thionin-stained tissue sections. The study establishes the reliability of a linear-motor based device for producing repeatable damage in a CCI model, demonstrates the power of longitudinal MRI in studying damage evolution, and confirms that a simple battery of functional tests record sensorimotor impairment and recovery.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Brain Injuries / pathology
  • Brain Injuries / physiopathology*
  • Cerebral Cortex / injuries*
  • Cerebral Cortex / pathology
  • Cerebral Cortex / physiopathology*
  • Denervation / instrumentation
  • Denervation / methods
  • Disability Evaluation
  • Disease Models, Animal*
  • Disease Progression
  • Electronics, Medical / instrumentation
  • Electronics, Medical / methods*
  • Longitudinal Studies
  • Magnetic Resonance Imaging / methods*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Motor Cortex / injuries
  • Motor Cortex / pathology
  • Motor Cortex / physiopathology
  • Movement Disorders / etiology
  • Movement Disorders / pathology
  • Movement Disorders / physiopathology
  • Nerve Degeneration / etiology
  • Nerve Degeneration / pathology
  • Nerve Degeneration / physiopathology
  • Predictive Value of Tests
  • Recovery of Function / physiology