The structure of the zetazeta transmembrane dimer reveals features essential for its assembly with the T cell receptor

Cell. 2006 Oct 20;127(2):355-68. doi: 10.1016/j.cell.2006.08.044.


The T cell receptor (TCR) alphabeta heterodimer communicates ligand binding to the cell interior via noncovalently associated CD3gammaepsilon, CD3deltaepsilon, and zetazeta dimers. While structures of extracellular components of the TCR-CD3 complex are known, the transmembrane (TM) domains that mediate assembly have eluded structural characterization. Incorporation of the zetazeta signaling module is known to require one basic TCRalpha and two zetazeta aspartic acid TM residues. We report the NMR structure of the zetazeta(TM) dimer, a left-handed coiled coil with substantial polar contacts. Mutagenesis experiments demonstrate that three polar positions are critical for zetazeta dimerization and assembly with TCR. The two aspartic acids create a single structural unit at the zetazeta interface stabilized by extensive hydrogen bonding, and there is evidence for a structural water molecule (or molecules) within close proximity. This structural unit, representing only the second transmembrane dimer interface solved to date, serves as a paradigm for the assembly of all modules involved in TCR signaling.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Aspartic Acid / chemistry
  • Dimerization
  • Humans
  • Hydrogen Bonding
  • Membrane Proteins / chemistry*
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism
  • Models, Molecular
  • Molecular Sequence Data
  • Mutagenesis
  • Nuclear Magnetic Resonance, Biomolecular
  • Peptides / chemistry
  • Peptides / metabolism
  • Protein Binding
  • Protein Conformation
  • Protein Engineering
  • Protein Structure, Tertiary
  • Receptor-CD3 Complex, Antigen, T-Cell / chemistry*
  • Receptor-CD3 Complex, Antigen, T-Cell / metabolism
  • Receptors, Antigen, T-Cell / chemistry*
  • Receptors, Antigen, T-Cell / genetics
  • Receptors, Antigen, T-Cell / metabolism
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism


  • Membrane Proteins
  • Peptides
  • Receptor-CD3 Complex, Antigen, T-Cell
  • Receptors, Antigen, T-Cell
  • Recombinant Proteins
  • antigen T cell receptor, zeta chain
  • Aspartic Acid

Associated data

  • PDB/2HAC