Abstract
FLT3 mutations and cell-surface antigen were investigated in 29 DR-negative (DR(-)) M1/M2 AML samples in comparison with 30 DR-positive (DR(+)) M1/M2 AML samples. FLT3-ITD was detected in 59.3% and D835 was detected in 7.4% of the samples. The incidence of FLT3-ITD was higher in the DR(-) group (59.3%) than in the DR(+) group (17.9%; P=0.002). The DR(-) status was associated with the CD34(-) (82.8%), CD7(-) (92.9%) and CD45RO(+) status (76%). Our results indicated that FLT3 mutation is the most common gene alteration found in the DR(-) M1/M2 AML. These results are important for further characterizing this phenotypic AML entity.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Antigens, CD34 / metabolism
-
Antigens, CD7 / metabolism
-
Antigens, Surface / metabolism*
-
HLA-DR Antigens / metabolism*
-
Humans
-
Karyotyping
-
Leukemia, Myeloid, Acute / genetics*
-
Leukemia, Myeloid, Acute / metabolism
-
Leukocyte Common Antigens / metabolism
-
Molecular Sequence Data
-
Mutation / genetics*
-
Oncogene Proteins, Fusion / genetics
-
Oncogene Proteins, Fusion / metabolism
-
RNA, Messenger / genetics
-
RNA, Messenger / metabolism
-
RNA, Neoplasm / genetics
-
RNA, Neoplasm / metabolism
-
Reverse Transcriptase Polymerase Chain Reaction
-
Sequence Homology, Amino Acid
-
fms-Like Tyrosine Kinase 3 / genetics*
-
fms-Like Tyrosine Kinase 3 / metabolism
Substances
-
Antigens, CD34
-
Antigens, CD7
-
Antigens, Surface
-
HLA-DR Antigens
-
Oncogene Proteins, Fusion
-
RNA, Messenger
-
RNA, Neoplasm
-
promyelocytic leukemia-retinoic acid receptor alpha fusion oncoprotein
-
FLT3 protein, human
-
fms-Like Tyrosine Kinase 3
-
Leukocyte Common Antigens