Sequence analysis of the rfb loci, encoding proteins involved in the biosynthesis of the Salmonella enterica O17 and O18 antigens: serogroup-specific identification by PCR

Collette Fitzgerald; Linda Gheesling; Marcus Collins; Patricia I Fields

doi:10.1128/AEM.01046-06

Sequence analysis of the rfb loci, encoding proteins involved in the biosynthesis of the Salmonella enterica O17 and O18 antigens: serogroup-specific identification by PCR

Appl Environ Microbiol. 2006 Dec;72(12):7949-53. doi: 10.1128/AEM.01046-06. Epub 2006 Oct 20.

Authors

Collette Fitzgerald¹, Linda Gheesling, Marcus Collins, Patricia I Fields

Affiliation

¹ Foodborne and Diarrheal Diseases Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Atlanta, GA 30333, USA. chf3@cdc.gov

Abstract

We report sequencing of the O antigen encoded by the rfb gene cluster of Salmonella enterica serotype Jangwani (O17) and Salmonella serotype Cerro (O18). We developed serogroup O17- and O18-specific PCR assays based on rfb gene targets and found them to be sensitive and specific for rapid identification of Salmonella serogroups O17 and O18.

MeSH terms

Bacterial Proteins / genetics*
Bacterial Typing Techniques
Humans
Molecular Sequence Data
Multigene Family
O Antigens / biosynthesis*
Polymerase Chain Reaction / methods*
Salmonella enterica / classification*
Salmonella enterica / genetics
Salmonella enterica / immunology
Sequence Analysis, DNA*
Serotyping
Species Specificity

Substances

Bacterial Proteins
O Antigens
rfb protein, Bacteria

Associated data

GENBANK/EF032634
GENBANK/EF032635