FRIGIDA LIKE 2 Is a Functional Allele in Landsberg Erecta and Compensates for a Nonsense Allele of FRIGIDA LIKE 1

Plant Physiol. 2006 Dec;142(4):1728-38. doi: 10.1104/pp.106.085571. Epub 2006 Oct 20.

Abstract

The Landsberg erecta (Ler) accession of Arabidopsis (Arabidopsis thaliana) has a weak allele of the floral inhibitor FLOWERING LOCUS C (FLC). FLC-Ler is weakly up-regulated by the active San Feliu-2 (Sf2) allele of FRIGIDA (FRI-Sf2), resulting in a moderately late-flowering phenotype. By contrast, the Columbia (Col) allele of FLC is strongly up-regulated by FRI-Sf2, resulting in a very late-flowering phenotype. In Col, the FRI-related gene FRI LIKE 1 (FRL1) is required for FRI-mediated up-regulation of FLC. It is shown here that in Ler, the FRL1-related gene FRI LIKE 2 (FRL2), but not FRL1, is required for FRI-mediated up-regulation of FLC. FRL1-Ler is shown to be a nonsense allele of FRL1 due to a naturally occurring premature stop codon in the middle of the conceptual protein sequence, suggesting that FRL1-Ler is nonfunctional. Compared to FRL2-Col, FRL2-Ler has two amino acid changes in the conceptual protein sequence. Plants homozygous for FRI-Sf2, FLC-Ler, FRL1-Ler, and FRL2-Col have no detectable FLC expression, resulting in an extremely early flowering phenotype. Transformation of a genomic fragment of FRL2-Ler, but not of FRL2-Col, into a recombinant inbred line derived from these plants restores both FRI-mediated up-regulation of FLC expression and a late-flowering phenotype, indicating that FRL2-Ler is the functional allele of FRL2. Taken together, these results suggest that in the two different Arabidopsis accessions Col and Ler, either FRL1 or FRL2, but not both, is functional and required for FRI-mediated up-regulation of FLC.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Alleles*
  • Amino Acid Sequence
  • Arabidopsis / genetics
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism
  • Arabidopsis Proteins / physiology*
  • Codon, Nonsense
  • Flowers / genetics
  • Flowers / growth & development
  • Flowers / metabolism
  • Gene Expression Regulation, Plant
  • MADS Domain Proteins / genetics
  • MADS Domain Proteins / metabolism
  • Molecular Sequence Data
  • Phenotype
  • Photoperiod
  • Sequence Alignment

Substances

  • Arabidopsis Proteins
  • Codon, Nonsense
  • FLF protein, Arabidopsis
  • FRL1 protein, Arabidopsis
  • FRL2 protein, Arabidopsis
  • MADS Domain Proteins