Basal Cell-Specific and Hyperproliferation-Related Keratins in Human Breast Cancer

Am J Pathol. 1991 Mar;138(3):751-63.

Abstract

In normal breast tissue and in noninvasive breast carcinomas, various keratin-14 antibodies were reactive predominantly with the basal/myoepithelial cell layer, although mainly in terminal and larger ducts luminal cells sometimes also were stained. A similar reaction pattern was found with an antibody directed against keratin 17, although this antibody was more often found negative than keratin 14 in the pre-existing myoepithelial cells in intraductal carcinomas. Furthermore antibodies reactive with hyperproliferation-related keratins 6 and 16 were used. One of these (LL025) was completely negative in normal breast tissue and noninvasive breast carcinomas. However 10% of the invasive carcinomas were diffusely or focally positive with this latter antibody, while in 18 of 115 cases of invasive breast carcinomas studied, a basal cell phenotype was detected. A relatively high concordance was found between the carcinomas immunostaining with the basal cell and the hyperproliferation-related keratins, but not between these markers and the proliferation marker Ki-67. This supports the conclusion that basal cells in breast cancer may show extensive proliferation, and that absence of Ki-67 staining does not mean that (tumor) cells are not proliferating.

MeSH terms

  • Antibodies, Monoclonal
  • Biomarkers, Tumor
  • Breast / metabolism
  • Breast Neoplasms / metabolism*
  • Breast Neoplasms / pathology
  • Carcinoma / metabolism*
  • Carcinoma / pathology
  • Carcinoma in Situ / metabolism*
  • Carcinoma in Situ / pathology
  • Carcinoma, Intraductal, Noninfiltrating / metabolism*
  • Carcinoma, Intraductal, Noninfiltrating / pathology
  • Cell Division
  • Female
  • Humans
  • Keratins / metabolism*
  • Keratins / physiology
  • Neoplasm Invasiveness
  • Reference Values

Substances

  • Antibodies, Monoclonal
  • Biomarkers, Tumor
  • Keratins