Gafchromic EBT film, a new high sensitivity radiochromic film has been tested for variations in optical properties due to scanning orientation. Gafchromic EBT film has been shown to produce a scanning orientation effect whereby variations in measured relative optical density are found due to the films orientation relative to the scanner direction. This relative optical density change was found to be relatively consistent for different films exposed to varying dose levels ranging from 0 Gy to 3 Gy. A maximum variation of 0.0157 +/- 0.0035 in optical density (OD) was found. This relates to an approximate 15% variation in net OD for a 50 cGy irradiated film and 4% variation for a 3 Gy irradiated film. No noticeable effects or variations were seen with changing scanning resolution or with the film placed "up or down" during scanning. Other Gafchromic film types were tested and compared to EBT for unirradiated film to assess the magnitude of this orientation effect on the scanner used and results showed that EBT produced a significantly higher effect that MD-55-2, HS, XR type T and XR type R film by up to 3 times. As such, providing the same orientation of EBT film when scanning for dosimetric analysis becomes an essential part of EBT film dosimetry.