Evaluation of 3-carboxy-4(1H)-quinolones as inhibitors of human protein kinase CK2

J Med Chem. 2006 Nov 2;49(22):6443-50. doi: 10.1021/jm050048t.


Due to the emerging role of protein kinase CK2 as a molecule that participates not only in the development of some cancers but also in viral infections and inflammatory failures, small organic inhibitors of CK2, besides application in scientific research, may have therapeutic significance. In this paper, we present a new class of CK2 inhibitors-3-carboxy-4(1H)-quinolones. This class of inhibitors has been selected via receptor-based virtual screening of the Otava compound library. It was revealed that the most active compounds, 5,6,8-trichloro-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (7) (IC(50) = 0.3 microM) and 4-oxo-1,4-dihydrobenzo[h]quinoline-3-carboxylic acid (9) (IC(50) = 1 microM), are ATP competitive (K(i) values are 0.06 and 0.28 microM, respectively). Evaluation of the inhibitors on seven protein kinases shows considerable selectivity toward CK2. According to theoretical calculations and experimental data, a structural model describing the key features of 3-carboxy-4(1H)-quinolones responsible for tight binding to CK2 active site has been developed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Casein Kinase II / antagonists & inhibitors*
  • Computer Simulation
  • Drug Evaluation, Preclinical
  • Enzyme Inhibitors / chemical synthesis*
  • Enzyme Inhibitors / pharmacology*
  • Hydrogen Bonding
  • Indicators and Reagents
  • Ligands
  • Models, Molecular
  • Quinolones / chemical synthesis*
  • Quinolones / pharmacology*
  • Receptors, Drug / chemistry
  • Receptors, Drug / genetics
  • Recombinant Proteins
  • Software
  • Structure-Activity Relationship
  • Substrate Specificity


  • Enzyme Inhibitors
  • Indicators and Reagents
  • Ligands
  • Quinolones
  • Receptors, Drug
  • Recombinant Proteins
  • Adenosine Triphosphate
  • Casein Kinase II